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bst dna polymerase large fragment  (New England Biolabs)


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    New England Biolabs bst dna polymerase large fragment
    Bst Dna Polymerase Large Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 783 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bst+dna+polymerase+large+fragment/Bst+DNA+Polymerase%2C+Large+Fragment/10__3390_slash_jof12050351-69-22-27
    Average 97 stars, based on 783 article reviews
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    Related Articles

    other:

    Article Title: A reverse transcription-free CRISPR/Cas12a biosensor for ultrasensitive detection of SARS-CoV-2 variants.
    Article Snippet: In this work, we developed a reverse transcription-free self-primer isothermal exponential amplification reaction (RTF-SP-EXPAR) combined with a CRISPR/Cas12a biosensor for the simultaneous detection of singleand double-stranded amplification products of SARS-CoV-2 mutant variants.. SP-EXPAR can recognize RNA targets directly, simultaneously generate double-stranded DNA products containing PAM sequences, and single-stranded DNA products without PAM sequences.. Both types of SP-EXPAR products can be recognized by the crRNA and produce fluorescent signals, thereby enhancing detection sensitivity.

    Article Title: Preliminary  Validation of a Colorimetric Loop-Mediated Isothermal Amplification (c-LAMP) Assay for Detection of Pythium insidiosum in Clinical Specimens
    Article Snippet: In brief, each 25 μL reaction included the following components: 1× ThermoPol buffer, 6 mM MgSO4, 1.4 mM dNTPs, 8 U of Bst DNA polymerase large fragment (New England Biolabs Inc., Ipswich, MA, USA), 0.4 M betaine (Sigma-Aldrich, St. Louis, MO, USA), and an ITS primer mix consisting of 0.2 μM F3 (5′- GGCAGAATGTGAGGTGTCTC-3′) and B3 (5′-GGAAACAACACCCCGTCAG-3′), 1.6 μM FIP (5′-ACAGATCACTGCGTTCGAGCAT-TTTT-GGAGATAGCACGAGTCCCT-3′) and BIP (5′-TCAGATTGCTTTGCGCTGGTGG-TTTT-CCGAAGCCTAACATACCGC-3′), and 0.4 μM LF (5′-GACACAAGAGAGATCAACGTACATT-3′) and LB (5′-AGGACATTAAGGA GATGACCTCTAT-3′) (Bio Basic Inc., Markham, ON, Canada).

    Sequencing:

    Article Title: Heterogeneity between VIP and GRP neurons underlies AVP receptor signaling in the mouse suprachiasmatic nucleus.
    Article Snippet: .. A set of gene-specific probes for the mouse V1a mRNA (NM_016847) was designed using the OligoMiner pipeline46 followed by BLAST homology search to ensure minimal sequence-similarity to other transcripts (probe sequences are available in Supplementary Data 2). ssDNA probes were extended by primer exchange reaction (PER)25, using BST DNA polymerase large fragment (New England Biolabs), dHTP (dATP, dTTP and dCTP), DNA hairpin oligos, and Clean.G oligo, according to a protocol by Kishi et al25. .. Secondary DIG-labeled PER concatemers targeting the primary probes were extended using DIG-11-dUTP (Roche Diagnostics) for anti-DIG-POD-based detection using StyramideTM Signal Amplification system (AAT Bioquest) (see Supplementary Data 2 for sequences of oligos used for SABER-FISH).

    Article Title: Heterogeneity between VIP and GRP neurons underlies AVP receptor signaling in the mouse suprachiasmatic nucleus
    Article Snippet: .. A set of gene-specific probes for the mouse V1a mRNA ( NM_016847 ) was designed using the OligoMiner pipeline followed by BLAST homology search to ensure minimal sequence-similarity to other transcripts (probe sequences are available in Supplementary Data ). ssDNA probes were extended by primer exchange reaction (PER) , using BST DNA polymerase large fragment (New England Biolabs), dHTP (dATP, dTTP and dCTP), DNA hairpin oligos, and Clean.G oligo, according to a protocol by Kishi et al . .. Secondary DIG-labeled PER concatemers targeting the primary probes were extended using DIG-11-dUTP (Roche Diagnostics) for anti-DIG-POD-based detection using StyramideTM Signal Amplification system (AAT Bioquest) (see Supplementary Data for sequences of oligos used for SABER-FISH).

    Incubation:

    Article Title: Spatiotemporal Regulation of DNA Condensate Formation Through Molecular Reaction Network
    Article Snippet: The gel was observed on an imaging device (FasDigi Compact, Nippon Genetics, Tokyo, Japan). .. Enzymatic Reactions To confirm that the conversion reaction induces the condensate formation, 0 or 1 μM T, 0 or 1 μM C, 80 units/ml Bst DNA polymerase large fragment (New England Biolabs), and assembled biSE Y-motif were mixed in the reaction buffer (the folding buffer with 0.5 mg/ml BSA and 4 mM DTT) and incubated at 45°C until observation. .. To confirm the inhibition reaction, 2 μM T, 2 μM A, 1 μM C, 80 units/ml Bst polymerase, 200 units/μl Nb.BsmI nicking endonuclease (New England Biolabs), ttRecJ exonucleas, and the assembled bi-SE Y-motif were mixed in the buffer with the addtition of 0, 0.5, or 1 μM of I.

    Article Title: Spatiotemporal Regulation of DNA Condensate Formation Through Molecular Reaction Network
    Article Snippet: The nondenaturing 8% (w/w) polyacrylamide gels were prepared by mixing 2 ml of 40% (w/v) acrylamide/bis (29/1) solution (Nacalai Tesque, Kyoto, Japan) and 1 ml of 10 × Tris-Borate EDTA (TBE) buffer (TaKaRa Bio, Kusatsu, Japan) with ultrapure water (total volume was 10 ml), followed by the addition of 100 μl of 10% APS and 10 μl of TEMED for gelation. .. To confirm the conversion reaction, 0.1 μM T, 0.1 μM C, and 80 units/ml Bst DNA polymerase large fragment (New England Biolabs) were mixed in the reaction buffer (the folding buffer with 0.5 mg/ ml BSA and 4 mM DTT) and incubated for 0, 1, 2, and 3 h. After the incubation, sample solutions were placed at 80°C for 10 min for heat inactivation of Bst polymerase and then cooled to 25°C at a rate of –1°C/min. .. PAGE was performed with 1 × TBE buffer with 5 mM MgCl2 under a constant voltage of 100 V, using an electrophoresis power supply (BP-TN30, BIO-CRAFT) for 60 min. After electrophoresis, the polyacrylamide gel was stained with 1 × TBE buffer containing 1 × SYBR Gold (Thermo Fisher Scientific, Waltham, MA, USA) for 10 min.

    Ligation:

    Article Title: Sequencing by structure assembly
    Article Snippet: .. After ligation, the 5′ overhang ends are filled-in using a DNA polymerase with, such as Bst DNA polymerase large fragment (NEB #M0275L). ..

    Reverse Transcription:

    Article Title: Isolation of cells in a nanopore sensor array
    Article Snippet: .. Polymerases include, but are not limited to, DNA-dependent DNA polymerases, DNA-dependent RNA polymerases, RNA-dependent DNA polymerases, RNA-dependent RNA polymerases, T7 DNA polymerase, T3 DNA polymerase, T4 DNA polymerase, T7 RNA polymerase, T3 RNA polymerase, SP6 RNA polymerase, DNA polymerase I, Klenow fragment, Thermophilus aquaticus DNA polymerase, Tth DNA polymerase, VentR® DNA polymerase (New England Biolabs), Deep VentR® DNA polymerase (New England Biolabs), Bst DNA Polymerase Large Fragment, Stoeffel Fragment, 90N DNA Polymerase, 90N DNA polymerase, Pfu DNA Polymerase, TfI DNA Polymerase, Tth DNA Polymerase, RepliPHI Phi29 Polymerase, TIi DNA polymerase, eukaryotic DNA polymerase beta, telomerase, TherminatorTM polymerase (New England Biolabs), KOD HiFiTM DNA polymerase (Novagen), KOD1 DNA polymerase, Q-beta replicase, terminal transferase, AMV reverse transcriptase, M-MLV reverse transcriptase, Phi6 reverse transcriptase, HIV-1 reverse transcriptase, novel polymerases discovered by bioprospecting, and polymerases cited in US 2007/0048748, U.S. Pat. ..



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    (a) Principle of proximity detection by in situ PER (left) and reaction mechanism (right). The docking sites for primer and hairpin need to be in close physical proximity for the catalytic primer extension to take place. Extended DNA concatemer is detected by fluorescently labeled imager oligos. When a cognate primer and hairpin pair are in close proximity, PER – takes place. The <t>polymerase</t> extends the primer with copies of sequence a and generates a linear ssDNA concatemer through autonomous and isothermal cycles of: (i) primer-hairpin binding, (ii) extension of the primer by a strand-displacing polymerase (in this work BST large fragment) using the hairpin’s a* sequence as a template, (iii) branch migration, (iv) dissociation of the primer from the hairpin. (b) Applications for proximity detection by ProPER. Primer and hairpin are docked to RNA by in situ hybridization of DNA probes or to proteins via DNA-labeled antibodies to detect proximity of the molecules or coincidence of probes on the same target. Bottom: In detection cascades different sequences are attached in a programmed order. Right: The life cycle of an RNA can be revealed by multiplexed detection of proximity to life-cycle markers. (c) In situ RNA FISH and ProPER for CBX5 gene in HeLa cells under limiting probe numbers. SABER-FISH was performed with in vitro pre-extended concatemers, whereas for ProPER primers were extended in situ after docking to a single site (ProPER medium) or multiple sites on a pre-extended primary probe (ProPER high). Representative images show maximum projections of FISH/ProPER signal (orange to white gradient) and DAPI (cyan). (d) Negative controls for the ProPER medium condition, where primer, hairpin dock or polymerase were left out. I Raw signal intensity of the spots (in arbitrary fluorescence units, a.u.) and (f) spot counts per cell showed higher values for ProPER compared to SABER-FISH with a small number of FISH probes (5-6 oligo probes). Signal to background ratio (S/B) is noted above each curve (see Methods ). Mann-Whitney-Wilcoxon test, two-sided, * p<0.05, *** p<0.001 (exact p-values in Supplementary Table S3 ). N=105-782 cells per condition. Scale bars 10 μm.
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    (a) Principle of proximity detection by in situ PER (left) and reaction mechanism (right). The docking sites for primer and hairpin need to be in close physical proximity for the catalytic primer extension to take place. Extended DNA concatemer is detected by fluorescently labeled imager oligos. When a cognate primer and hairpin pair are in close proximity, PER – takes place. The <t>polymerase</t> extends the primer with copies of sequence a and generates a linear ssDNA concatemer through autonomous and isothermal cycles of: (i) primer-hairpin binding, (ii) extension of the primer by a strand-displacing polymerase (in this work BST large fragment) using the hairpin’s a* sequence as a template, (iii) branch migration, (iv) dissociation of the primer from the hairpin. (b) Applications for proximity detection by ProPER. Primer and hairpin are docked to RNA by in situ hybridization of DNA probes or to proteins via DNA-labeled antibodies to detect proximity of the molecules or coincidence of probes on the same target. Bottom: In detection cascades different sequences are attached in a programmed order. Right: The life cycle of an RNA can be revealed by multiplexed detection of proximity to life-cycle markers. (c) In situ RNA FISH and ProPER for CBX5 gene in HeLa cells under limiting probe numbers. SABER-FISH was performed with in vitro pre-extended concatemers, whereas for ProPER primers were extended in situ after docking to a single site (ProPER medium) or multiple sites on a pre-extended primary probe (ProPER high). Representative images show maximum projections of FISH/ProPER signal (orange to white gradient) and DAPI (cyan). (d) Negative controls for the ProPER medium condition, where primer, hairpin dock or polymerase were left out. I Raw signal intensity of the spots (in arbitrary fluorescence units, a.u.) and (f) spot counts per cell showed higher values for ProPER compared to SABER-FISH with a small number of FISH probes (5-6 oligo probes). Signal to background ratio (S/B) is noted above each curve (see Methods ). Mann-Whitney-Wilcoxon test, two-sided, * p<0.05, *** p<0.001 (exact p-values in Supplementary Table S3 ). N=105-782 cells per condition. Scale bars 10 μm.
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    (a) Principle of proximity detection by in situ PER (left) and reaction mechanism (right). The docking sites for primer and hairpin need to be in close physical proximity for the catalytic primer extension to take place. Extended DNA concatemer is detected by fluorescently labeled imager oligos. When a cognate primer and hairpin pair are in close proximity, PER – takes place. The <t>polymerase</t> extends the primer with copies of sequence a and generates a linear ssDNA concatemer through autonomous and isothermal cycles of: (i) primer-hairpin binding, (ii) extension of the primer by a strand-displacing polymerase (in this work BST large fragment) using the hairpin’s a* sequence as a template, (iii) branch migration, (iv) dissociation of the primer from the hairpin. (b) Applications for proximity detection by ProPER. Primer and hairpin are docked to RNA by in situ hybridization of DNA probes or to proteins via DNA-labeled antibodies to detect proximity of the molecules or coincidence of probes on the same target. Bottom: In detection cascades different sequences are attached in a programmed order. Right: The life cycle of an RNA can be revealed by multiplexed detection of proximity to life-cycle markers. (c) In situ RNA FISH and ProPER for CBX5 gene in HeLa cells under limiting probe numbers. SABER-FISH was performed with in vitro pre-extended concatemers, whereas for ProPER primers were extended in situ after docking to a single site (ProPER medium) or multiple sites on a pre-extended primary probe (ProPER high). Representative images show maximum projections of FISH/ProPER signal (orange to white gradient) and DAPI (cyan). (d) Negative controls for the ProPER medium condition, where primer, hairpin dock or polymerase were left out. I Raw signal intensity of the spots (in arbitrary fluorescence units, a.u.) and (f) spot counts per cell showed higher values for ProPER compared to SABER-FISH with a small number of FISH probes (5-6 oligo probes). Signal to background ratio (S/B) is noted above each curve (see Methods ). Mann-Whitney-Wilcoxon test, two-sided, * p<0.05, *** p<0.001 (exact p-values in Supplementary Table S3 ). N=105-782 cells per condition. Scale bars 10 μm.
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    (a) Principle of proximity detection by in situ PER (left) and reaction mechanism (right). The docking sites for primer and hairpin need to be in close physical proximity for the catalytic primer extension to take place. Extended DNA concatemer is detected by fluorescently labeled imager oligos. When a cognate primer and hairpin pair are in close proximity, PER – takes place. The <t>polymerase</t> extends the primer with copies of sequence a and generates a linear ssDNA concatemer through autonomous and isothermal cycles of: (i) primer-hairpin binding, (ii) extension of the primer by a strand-displacing polymerase (in this work BST large fragment) using the hairpin’s a* sequence as a template, (iii) branch migration, (iv) dissociation of the primer from the hairpin. (b) Applications for proximity detection by ProPER. Primer and hairpin are docked to RNA by in situ hybridization of DNA probes or to proteins via DNA-labeled antibodies to detect proximity of the molecules or coincidence of probes on the same target. Bottom: In detection cascades different sequences are attached in a programmed order. Right: The life cycle of an RNA can be revealed by multiplexed detection of proximity to life-cycle markers. (c) In situ RNA FISH and ProPER for CBX5 gene in HeLa cells under limiting probe numbers. SABER-FISH was performed with in vitro pre-extended concatemers, whereas for ProPER primers were extended in situ after docking to a single site (ProPER medium) or multiple sites on a pre-extended primary probe (ProPER high). Representative images show maximum projections of FISH/ProPER signal (orange to white gradient) and DAPI (cyan). (d) Negative controls for the ProPER medium condition, where primer, hairpin dock or polymerase were left out. I Raw signal intensity of the spots (in arbitrary fluorescence units, a.u.) and (f) spot counts per cell showed higher values for ProPER compared to SABER-FISH with a small number of FISH probes (5-6 oligo probes). Signal to background ratio (S/B) is noted above each curve (see Methods ). Mann-Whitney-Wilcoxon test, two-sided, * p<0.05, *** p<0.001 (exact p-values in Supplementary Table S3 ). N=105-782 cells per condition. Scale bars 10 μm.
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    large fragment bst dna polymerase  (New England Biolabs)
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    (a) Principle of proximity detection by in situ PER (left) and reaction mechanism (right). The docking sites for primer and hairpin need to be in close physical proximity for the catalytic primer extension to take place. Extended DNA concatemer is detected by fluorescently labeled imager oligos. When a cognate primer and hairpin pair are in close proximity, PER – takes place. The <t>polymerase</t> extends the primer with copies of sequence a and generates a linear ssDNA concatemer through autonomous and isothermal cycles of: (i) primer-hairpin binding, (ii) extension of the primer by a strand-displacing polymerase (in this work BST large fragment) using the hairpin’s a* sequence as a template, (iii) branch migration, (iv) dissociation of the primer from the hairpin. (b) Applications for proximity detection by ProPER. Primer and hairpin are docked to RNA by in situ hybridization of DNA probes or to proteins via DNA-labeled antibodies to detect proximity of the molecules or coincidence of probes on the same target. Bottom: In detection cascades different sequences are attached in a programmed order. Right: The life cycle of an RNA can be revealed by multiplexed detection of proximity to life-cycle markers. (c) In situ RNA FISH and ProPER for CBX5 gene in HeLa cells under limiting probe numbers. SABER-FISH was performed with in vitro pre-extended concatemers, whereas for ProPER primers were extended in situ after docking to a single site (ProPER medium) or multiple sites on a pre-extended primary probe (ProPER high). Representative images show maximum projections of FISH/ProPER signal (orange to white gradient) and DAPI (cyan). (d) Negative controls for the ProPER medium condition, where primer, hairpin dock or polymerase were left out. I Raw signal intensity of the spots (in arbitrary fluorescence units, a.u.) and (f) spot counts per cell showed higher values for ProPER compared to SABER-FISH with a small number of FISH probes (5-6 oligo probes). Signal to background ratio (S/B) is noted above each curve (see Methods ). Mann-Whitney-Wilcoxon test, two-sided, * p<0.05, *** p<0.001 (exact p-values in Supplementary Table S3 ). N=105-782 cells per condition. Scale bars 10 μm.
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    bst large fragment dna polymerase  (New England Biolabs)
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    New England Biolabs bst large fragment dna polymerase
    (a) Principle of proximity detection by in situ PER (left) and reaction mechanism (right). The docking sites for primer and hairpin need to be in close physical proximity for the catalytic primer extension to take place. Extended DNA concatemer is detected by fluorescently labeled imager oligos. When a cognate primer and hairpin pair are in close proximity, PER – takes place. The <t>polymerase</t> extends the primer with copies of sequence a and generates a linear ssDNA concatemer through autonomous and isothermal cycles of: (i) primer-hairpin binding, (ii) extension of the primer by a strand-displacing polymerase (in this work BST large fragment) using the hairpin’s a* sequence as a template, (iii) branch migration, (iv) dissociation of the primer from the hairpin. (b) Applications for proximity detection by ProPER. Primer and hairpin are docked to RNA by in situ hybridization of DNA probes or to proteins via DNA-labeled antibodies to detect proximity of the molecules or coincidence of probes on the same target. Bottom: In detection cascades different sequences are attached in a programmed order. Right: The life cycle of an RNA can be revealed by multiplexed detection of proximity to life-cycle markers. (c) In situ RNA FISH and ProPER for CBX5 gene in HeLa cells under limiting probe numbers. SABER-FISH was performed with in vitro pre-extended concatemers, whereas for ProPER primers were extended in situ after docking to a single site (ProPER medium) or multiple sites on a pre-extended primary probe (ProPER high). Representative images show maximum projections of FISH/ProPER signal (orange to white gradient) and DAPI (cyan). (d) Negative controls for the ProPER medium condition, where primer, hairpin dock or polymerase were left out. I Raw signal intensity of the spots (in arbitrary fluorescence units, a.u.) and (f) spot counts per cell showed higher values for ProPER compared to SABER-FISH with a small number of FISH probes (5-6 oligo probes). Signal to background ratio (S/B) is noted above each curve (see Methods ). Mann-Whitney-Wilcoxon test, two-sided, * p<0.05, *** p<0.001 (exact p-values in Supplementary Table S3 ). N=105-782 cells per condition. Scale bars 10 μm.
    Bst Large Fragment Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (a) Principle of proximity detection by in situ PER (left) and reaction mechanism (right). The docking sites for primer and hairpin need to be in close physical proximity for the catalytic primer extension to take place. Extended DNA concatemer is detected by fluorescently labeled imager oligos. When a cognate primer and hairpin pair are in close proximity, PER – takes place. The polymerase extends the primer with copies of sequence a and generates a linear ssDNA concatemer through autonomous and isothermal cycles of: (i) primer-hairpin binding, (ii) extension of the primer by a strand-displacing polymerase (in this work BST large fragment) using the hairpin’s a* sequence as a template, (iii) branch migration, (iv) dissociation of the primer from the hairpin. (b) Applications for proximity detection by ProPER. Primer and hairpin are docked to RNA by in situ hybridization of DNA probes or to proteins via DNA-labeled antibodies to detect proximity of the molecules or coincidence of probes on the same target. Bottom: In detection cascades different sequences are attached in a programmed order. Right: The life cycle of an RNA can be revealed by multiplexed detection of proximity to life-cycle markers. (c) In situ RNA FISH and ProPER for CBX5 gene in HeLa cells under limiting probe numbers. SABER-FISH was performed with in vitro pre-extended concatemers, whereas for ProPER primers were extended in situ after docking to a single site (ProPER medium) or multiple sites on a pre-extended primary probe (ProPER high). Representative images show maximum projections of FISH/ProPER signal (orange to white gradient) and DAPI (cyan). (d) Negative controls for the ProPER medium condition, where primer, hairpin dock or polymerase were left out. I Raw signal intensity of the spots (in arbitrary fluorescence units, a.u.) and (f) spot counts per cell showed higher values for ProPER compared to SABER-FISH with a small number of FISH probes (5-6 oligo probes). Signal to background ratio (S/B) is noted above each curve (see Methods ). Mann-Whitney-Wilcoxon test, two-sided, * p<0.05, *** p<0.001 (exact p-values in Supplementary Table S3 ). N=105-782 cells per condition. Scale bars 10 μm.

    Journal: bioRxiv

    Article Title: ProPER: Programmable, multiplexed detection of molecular proximities and RNA life-cycle stages in situ

    doi: 10.64898/2026.04.24.718482

    Figure Lengend Snippet: (a) Principle of proximity detection by in situ PER (left) and reaction mechanism (right). The docking sites for primer and hairpin need to be in close physical proximity for the catalytic primer extension to take place. Extended DNA concatemer is detected by fluorescently labeled imager oligos. When a cognate primer and hairpin pair are in close proximity, PER – takes place. The polymerase extends the primer with copies of sequence a and generates a linear ssDNA concatemer through autonomous and isothermal cycles of: (i) primer-hairpin binding, (ii) extension of the primer by a strand-displacing polymerase (in this work BST large fragment) using the hairpin’s a* sequence as a template, (iii) branch migration, (iv) dissociation of the primer from the hairpin. (b) Applications for proximity detection by ProPER. Primer and hairpin are docked to RNA by in situ hybridization of DNA probes or to proteins via DNA-labeled antibodies to detect proximity of the molecules or coincidence of probes on the same target. Bottom: In detection cascades different sequences are attached in a programmed order. Right: The life cycle of an RNA can be revealed by multiplexed detection of proximity to life-cycle markers. (c) In situ RNA FISH and ProPER for CBX5 gene in HeLa cells under limiting probe numbers. SABER-FISH was performed with in vitro pre-extended concatemers, whereas for ProPER primers were extended in situ after docking to a single site (ProPER medium) or multiple sites on a pre-extended primary probe (ProPER high). Representative images show maximum projections of FISH/ProPER signal (orange to white gradient) and DAPI (cyan). (d) Negative controls for the ProPER medium condition, where primer, hairpin dock or polymerase were left out. I Raw signal intensity of the spots (in arbitrary fluorescence units, a.u.) and (f) spot counts per cell showed higher values for ProPER compared to SABER-FISH with a small number of FISH probes (5-6 oligo probes). Signal to background ratio (S/B) is noted above each curve (see Methods ). Mann-Whitney-Wilcoxon test, two-sided, * p<0.05, *** p<0.001 (exact p-values in Supplementary Table S3 ). N=105-782 cells per condition. Scale bars 10 μm.

    Article Snippet: Final concentrations in 200 μL reaction volume were 1× PBS, 480U/ml Bst LF polymerase (NEB, M0275M), 10 mM MgSO4, 0.6 mM dNTPs (dATP, dCTP, dTTP), 0.1 μM Clean.G hairpin (IDT), 0.6-1.0 μM hairpin and 0.75 μM primer.

    Techniques: In Situ, Labeling, Sequencing, Binding Assay, Migration, RNA In Situ Hybridization, In Vitro, Fluorescence, MANN-WHITNEY